Composite

Part:BBa_K590062:Design

Designed by: Casey Ager, Ingrid Swanson, Seth Sagulo, Austin Moon   Group: iGEM11_Washington   (2011-09-15)

Gli1-ADC HivC-AAR Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2926
    Illegal XhoI site found at 4294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal AgeI site found at 3025
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBrick plasmid pSB3K3

Source

This part was generated by using Gibson cloning. The F1 origin, lacI gene, and Lac operator and promoter were taken from K314103. The HIVC and Gli1 genes were taken from parts K165006 and K165007. ADC and AAR were from Synechococcus elongatus, codon-optimized for E. coli by GenScript.

References